Calculation of the isothermal transformation diagram from measurements with continuous cooling

For the general calculation of the transformation of steels the transformation diagram for isothermal transformation must be known. It is described, how from dilatometric measurements with continuous cooling the transformation at a constant temperature can be calculated. To do this, the simplex method must be used as iteration process to achieve sufficient precision. Even when measuring data are scattered around an ideal value by ±5% the transformation diagram for isothermal transformation can be calculated with considerable accuracy.     

Stabilization of local structures by {pi}-CH and aromatic-backbone amide interactions involving prolyl and aromatic residues

Weakly polar interactions between the side-chain aromatic ringsand hydrogens of backbone amides (Ar–HN) and CHn of aliphaticgroups (     

Simultaneous biohydrogen production and wastewater treatment based on the selective enrichment of the fermentation ecosystem

Biohydrogen production from synthetic wastewater as substrate was studied in anaerobic small scale batch reactors. Enriched anaerobic mixed consortia sampled from various environments were used as parent inocula to start the bioreactors. Selective enrichments were achieved by various physical and chemical pretreatments and changes in the microbial communities were monitored by metagenomic and molecular diagnostics approaches. Experimental data showed the feasibility of biohydrogen production using synthetic wastewater as substrate. The hydrogen generation capability of the different mixed consortia is clearly dependent on the pretreatment methods. The described approach opens the possibility for an alternative way towards simultaneous wastewater treatment and renewable energy generation.     

Possibilities of using near infrared reflectance/transmittance spectroscopy for determination of polymeric protein in wheat

Possibilities of using near‐infrared reflectance and near‐infrared transmittance (NIR/NIT) spectroscopic techniques for detecting differences in amount and size distribution of polymeric proteins in wheat were investigated. To evaluate whether differences in polymeric protein due to genetic or environmental variations were detectable by NIR/NIT techniques, wheat materials of different background were used. Size‐exclusion high‐performance liquid chromatography was applied to detect variation in polymeric protein. Partial least squares regression gave high R² values between many protein parameters and NIR/NIT spectra (particularly second‐derivative spectra of NIR 1100–2500 nm region) of flours, while no such relationship was found for whole wheat grains. Most and highest correlations were found for total amount of extractable and unextractable proteins and monomer/polymer protein ratio. Some positive relationships were found between percentage of total unextractable polymeric protein in the total polymeric protein and percentage of large unextractable polymeric protein in the total large polymeric protein and NIR/NIT spectra. Thus, it was possible to detect differences in polymeric proteins with NIR/NIT techniques. The highest amount of positive correlations between NIR/NIT spectra and protein parameters was found to be due to environmental influences. Some correlations were found for breeding lines with a broad variation in gluten strength and polymeric protein composition, while a more homogeneous sample showed less correlation. Thereby, detection of variation in amount and size distribution of polymeric protein due to cultivar differences with NIR/NIT methods might be difficult. Copyright © 2007 Society of Chemical Industry     

An Adaptive Generalized Transmission Protocol for Ad Hoc Networks

Wireless networking and group communication in combination allows groups of dispersed mobile users to collaborate. This paper presents AGENT, a medium access control (MAC) protocol that unifies point-to-point and multi-point transmission services to facilitate group communication in ad hoc networks. Analysis and experiments performed in a simulated ad hoc network demonstrate that AGENT exhibits reliable and stable performance with high spatial bandwidth reuse. Moreover, variation in the proportion of point-to-point and multi-point traffic is shown to have little impact on the overall performance of AGENT. Comparison with the other tested MAC protocols reveals that the performance of AGENT is superior, achieving higher channel utilization and lower access delay.     

The Interaction of Buckminsterfullerene with Gamma-Cyclodextrin

During the past few years many papers were published on the gamma-cyclodextrin—C₆₀ interaction. These described different procedures for the synthesis of a 2:1 inclusion complex, which can be prepared in solid form and exists in solution only in the presence of high cyclo-dextrin excess as a magenta coloured solution or as a yellowish brown colloid both being metastable. The magenta solution may be stable for half a year at room temperature, then its transformation into a yellowish colloidal solution along with the formation of a brown precipitate proceeds within a few weeks. On longer boiling of the solutions, the partial oxidation of fullerene with the formation of fullerol seems to be probable.     

Detection of human papillomavirus gene sequences in laryngeal tumors and premalignant changes by polymerase chain reaction

Human papillomavirus gene sequences have been detected in a number of malignant and benign tumours. Non-oncogenic types 6 and 11 are etiological factors of benign mucosal tumours. Types 16 and 18 can be detected in malignancies most often but their role in the etiopathogenesis of cancers is still unclear. In our study we examined formalin-fixed and paraffin-embedded archive laryngeal tissues containing squamous cell carcinoma, papilloma and precancerous lesions for the presence of human papillomavirus genes. As a control we also examined tissues harbouring laryngeal nodules which represented the normal larynx in our study. After DNA preparation from the paraffin blocks we performed polymerase chain reaction to detect the DNA of human papillomavirus types 6, 11, 16 and 18. In the squamous cell carcinomas, papillomas and precancerous lesions the presence of human papillomavirus gene sequences was significantly higher than in the control group. To verify the integrity of DNA we also amplified a sequence deriving from the cellular beta-globin gene. Based on the 100% positivity for this gene we declare that the combination of our DNA preparation and polymerase chain reaction is a reliable method for detecting DNA sequences from formalin-fixed and paraffin-embedded tissues.     

Hydrogenases, accessory genes and the regulation of [NiFe] hydrogenase biosynthesis in Thiocapsa roseopersicina

The purple (Sulphur) phototrophic bacterium, Thiocapsa roseopersicina BBS contains several [NiFe] hydrogenases, of which two are membrane bound. Mutant T. roseopersicina cells, carrying deletions in both gene clusters showed hydrogenase activity. This activity was located in the cytoplasm. The structural gene cluster hoxEFUYH was identified and sequenced. In addition, genes homologous to hupUV/hoxBC, the hydrogen sensing hydrogenase have been identified and sequenced.Regulation of hydrogenase biosynthesis was studied in detail for HydSL (renamed HynSL). A random mutagenesis system was optimised for T. roseopersicina. One of the mutations was in a gene similar to that coding for the HypF proteins in other organisms. Inactivation of the hypF gene resulted in a 60-fold increase in hydrogen evolution under nitrogen fixing conditions. In addition to hypF, the following accessory genes were identified: hydD, hupK, hypC1, hypC2, hypDE. Characterisation of the corresponding gene products and search for additional accessory genes are in progress.     

A line clipping algorithm and its analysis

One of the classical problems of Computer Graphics: line clipping against a rectangle is revisited. Coordinate raster refinement and some unusual forms of the parametric equation of the line are used to develop formulae for a line clipping algorithm. The algorithm is first presented in a form, where clarity of presentation is the prime concern. It is then transformed into one big nested branch, which after optimisation is assumed to be the most efficient form with a heavy cost on size. It is assumed that any mathematical consideration of the clipping problem would after a similar optimisation lead to a branching structure of equal complexity and speed. Line clipping thus belongs to the class of problems for which after a proper mathematical and logical analysis automatic program transformations may do the rest. This work has been supported by a grant from the Hungarian Academy of Sciences, Project No. OTKA 2572/1991